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Next Generation Sequencing (NGS) Library Preparation Kits


A DNA library is a collection of DNA fragments that can be used for further molecular genetic investigations such as sequencing. A DNA library can represent a complete genome of an organism, parts of the genome, or a mixture of different organisms as used in microbiome analysis.
DNA library preparation is a crucial step in the next generation sequencing workflow. Library preparation can include various steps starting with nucleic acid extraction until template enrichment and sequencing. For some applications cDNA synthesis of RNA is needed prior DNA library preparation. In other applications a purification, selection or enrichment step of specific target RNA/ DNA is implemented. The library preparation workflow can vary greatly depending on the sample material, target nucleic acid type, NGS technology and application. In general library preparation can include target enrichment and amplification, DNA fragmentation, fragment size selection and multiple quality control steps as well as DNA fragment end repair, barcoding and sequencing adaptor ligation.
This website lists a number of different universal NGS library preparation kits as well as kits for different sequencing technologies such as Illumina, Ion Torrent or Roche454.
Furthermore, library preparation kits are listed according to different sample materials or applications such as cDNA, Chip-Seq, FFPE, blood, methyl sequencing, mRNA, or single cell sequencing.
Additionally, kits for barcoding, A-tailing and end repair are listed.


Sample Preparation |  Fragmentation  |  Fragment Size Selection  |  Quality Control  |  Library Preparation  |  Template Enrichtment/ Sequencing


Adaptor Ligation

Adaptor ligation is included in most library prep kits, if not ligase enzymes are listed here.


A-tailing/ DNA End Repair

NEBNext® dA-Tailing Module

The NEBNext® dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3´ end of a blunt DNA fragment (1). 3´-dA DNA tailing prevents concatamer formation during subsequent ligation steps. DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs.
Source: NEB

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NEBNext FFPE DNA Repair Mix

NEBNext FFPE DNA Repair Mix is a cocktail of enzymes formulated to repair DNA, and specifically optimized and validated for repair of FFPE DNA samples.
Source: NEB

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Barcoding

EpiNext™ NGS Barcode (Index) Set-12

The EpiNext™ NGS Barcode (Index) Set-12 is designed to construct multiplex DNA/RNA libraries used for next-generation sequencing with the Illumina platform including GAIIx, HiSeq, MiSeq, and NextSeq. This product includes 12 DNA barcodes, each of which contain an index and flow cell binding sequence and can be attached to the sample insert during library preparation. Multiplexing several samples in a single flow cell can be achieved by pooling with the EpiNext™ NGS Barcodes. Pooling barcoded samples into a single flow cell significantly reduces hands-on time and provides robust data quality in NGS.
Source: Epigentek

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GeneRead Adapter I Set A 12-plex (144)

For 144 reactions: 12 barcoded adapters for ligation to DNA library, for use with Illumina instruments
Source: Qiagen

GeneRead Adapter I Set B 12-plex (144)

For 144 reactions: 12 barcoded adapters for ligation to DNA library, for use with Illumina instruments

Source: Qiagen

GeneRead Adapter L Set 12-plex (72)

For 72 reactions: 12 barcoded adapters for ligation to DNA library, for use with Ion Torrent instruments
Source: Qiagen

Ion Xpress™ Barcode Adapter 1-96 Kit

The Ion Xpress™ Barcode Adapter 1-96 Kit provides an extensive set of 96 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, you may pool from 2 to 96 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the next generation sequencing workflow for a wide range of applications, including targeted resequencing.
Source: Thermo Fisher

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NEXTflex™ DNA Barcodes for Ion PGM™ and Ion Proton™

NEXTflex™ DNA Barcodes for Ion PGM™ and Ion Proton™ can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes use an indexed adapter with a 10 nt unique sequence, allowing for proper differentiation between samples and preventing poor reads caused by single base errors introduced during PCR. Up to 64 samples can be multiplexed using these adapters. The NEXTflex DNA Barcodes for Ion PGM and Ion Proton undergo stringent quality controls and are functionally validated by sequencing.
Source: BiooScientific

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SOLiD™ RNA Barcoding Kit

The SOLiD™ RNA Barcoding Kit consists of a set of PCR primers that are designed for use with the SOLiD™ Total RNA-Seq Kit or the SOLiD™ SAGE™ Kit with Barcoding Adaptor Module. Together, these combinations of kits enable multiplex sequencing for all RNA applications, including whole transcriptome, small RNA analysis, or SOLiD™ SAGE™.
Source: Thermo Fisher

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SOLiD™ Fragment Library Barcoding Kit 1–96

The SOLiD™ Fragment Library Barcoding Kit 1–96 provides a complete set of 96 unique barcode sequences for SOLiD v4.0. The kit includes all 6 individual SOLiD™ Fragment Library Barcoding Kit Modules containing 16 individually synthesized P2 adapters in each to make up the full set of 96 barcodes. For each unique barcode sufficient material is provided to label 8 µg of input genomic DNA, P1 adapter, and PCR Primer 1 and 2.
Source: Thermo Fisher

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General Library Preparation Kits

Encore® 384 Multiplex Library System

The Encore 384 Multiplex System is a commercial solution for deep multiplexing of NGS samples that expands NuGEN's current portfolio of Next Gen Sequencing library kits. The Encore 384 Multiplex System consists of a refined set of 384 molecularly "barcoded" library adaptors that enable deep multiplexing of sequencing samples within a library preparation system that is compatible with high-throughput automation.
Source: Nugen

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GeneRead Library Prep Kit

GeneRead Library Prep Kits provide an optimized and efficient workflow for library preparation in next-generation sequencing (NGS) applications. The kits allow fast and efficient preparation of DNA libraries that are for use on NGS platforms from Illumina and the Ion Torrent instrument from Life Technologies.
Source: Qiagen
Qiagen offers different solutions for library preparation.

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HaloPlexHS

HaloPlexHS is a high sensitivity amplicon-based targeted sequencing method based on the HaloPlex technology that incorporates molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Combined with the flexibility of panel customization up to 5Mb and an accelerated workflow of less than 6hr starting with 50ng gDNA, HaloPlexHS is well-suited for accurate rare variant detection in clinical research.
Source: Agilent

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KAPA HyperPlus kit

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube. Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows. Starting from 1ng DNA, library preparation within 2,5 hours.
Source: KAPABIOSYSTEMS

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KAPA HiFi

KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
Source: KAPABIOSYSTEMS

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KAPA Stranded RNA-Seq Kit

The KAPA Stranded RNA-Seq Kit with RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and low-bias library amplification, and include a streamlined, “with-bead” protocol.
Source: KAPABIOSYSTEMS

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NEXTflex Library Prep Kits

Bioo Scientific has automated a number of our NEXTflex Library Prep Kits on several major liquid handling platforms. Bioo Scientific’s NEXTflex automation protocols provide optimized workflows that require minimal user intervention for producing up to 96 libraries at a time. Automation protocols are available for the Beckman Biomek®FX and Biomek® FXP Laboratory Automation Workstations, the SciClone Workstations and the Tecan Freedom EVO Workstation.
Source: Bioo Scientific

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Ovation® Ultralow Library Systems V2

The Ovation Ultralow Library Systems V2 provide a simple, fast and scalable solution for producing libraries that can be used in a broad range of next-generation sequencing applications starting with nanogram amounts of DNA. The method used for adaptor ligation provides for low bias libraries suitable for RNA-Seq, Digital Gene Expression (DGE), genomic DNA sequencing, target capture, ChIP-Seq and more.
Source: Nugen

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Ovation® Rapid Library Systems

The Ovation Rapid Library Systems provide a simple, fast and scalable PCR-free solution for producing libraries used in next-generation sequencing. These systems enable library construction starting with as little as 100 ng of double-stranded DNA, without PCR amplification. The library construction workflow is suitable for a wide range of sequencing applications including RNA-Seq, Digital Gene Expression (DGE), genomic DNA sequencing, amplicon sequencing and more.
Source: Nugen

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Ovation Ultralow Library System

The Ovation Ultralow Library Systems V2 provide a simple, fast and scalable solution for producing libraries that can be used in a broad range of next-generation sequencing applications starting with 1 to 100 ng of DNA. The method used for adaptor ligation provides for low bias libraries suitable for RNA-Seq, Digital Gene Expression (DGE), genomic DNA sequencing, target capture, ChIP-Seq and more.
Source: Nugen

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Sentosa SQ Fragment Library Prep Kit

The Sentosa SQ Fragment Library Prep Kit is a ready-to-use kit for automated NGS DNA library preparation using the Sentosa SX101 instrument. The kit containsSentosa SQ Fragment Library Prep Reagents and Sentosa SQ Fragment Library Prep Solutions required to prepare up to 32 libraries from gDNAs (for whole genome sequencing), cDNAs or PCR amplicons (for amplicon sequencing) from the regions of interest within the genome, with DNA input size ranging from 350 bp to 35 Mbp. The Sentosa SQ Fragment Library Prep Kit protocol is automated on the SentosaSX101 platform.
Source: Vela Diagnostics

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Sciclone NGS/NGSx Consumable Kits A/ B or C

The NGS Consumables Kit A provides exactly what you need to run DNA sample preparation and DNA library preparation workflows for standard reagent platforms on the Sciclone® NGS workstation, taking the hassle out of buying NGS consumables.
The NGS Consumables Kit B provides exactly what you need to run RNA, stranded total RNA LT and mRNA LT sample preparation for standard reagent platforms on the Sciclone® NGS workstation, taking the hassle out of buying NGS consumables.
The NGS Consumables Kit C provides everything you need to run library preparation and target enrichment for standard reagent platforms on the Sciclone® NGS workstation, taking the hassle out of buying NGS consumables.
Source: PerkinElmer

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SureSeq NGS Library Preparation Kit

The SureSeq NGS Library Preparation Kit generates NGS libraries suitable for the capture of targeted genomic regions using hybridisation. Optimisation of enzymes and buffers enables the number of steps to be reduced, while still delivering libraries of the highest quality.
Source: Oxford Gene Technologies

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ThruPLEX DNA-seq Kit

ThruPLEX DNA-seq Kit builds on the innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA and increases sensitivity while providing up to 96 indexes for multiplexing. This kit can be used with fragmented DNA from any sample source – genomic, biofluids such as cell-free DNA, DNA from FFPE materials and cDNA. The entire ThruPLEX DNA-seq Kit workflow is performed in a single tube or well in about 2 hours and requires no purification steps or sample transfers.
Source: RUBICON GENOMICS

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SPARK™ DNA SAMPLE PREP KITS

SPARK™ DNA SAMPLE PREP KITS Preparation of library DNA is an important process required for generating valuable Next Generation Sequencing data. Enzymatics has developed lyophilized room temperature stable library construction kits for the Illumina® and Ion Torrent™ sequencing platforms. Each SPARK™ DNA Sample Prep kit provides lyophilized room temperature stable reaction master mixes for the preparation of adapter ligated DNA libraries for Next Generation Sequencing from sheared double-stranded DNA. The kits are designed for constructing genomic DNA libraries with 10 ng to 1000 ng of input DNA.
Source: Enzymatics/ Qiagen

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Library Preparation Kits for Ilumina

Accel-NGS 1S Plus DNA Library Kit

The Accel-NGS 1S Plus DNA Library Kit for Illumina enables users to make libraries from degraded and damaged DNA because innovative Swift technology makes library molecules from single-stranded DNA.
Source: Swift biosciences

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Accel-NGS 2S DNA Library Kit

The Accel-NGS 2S DNA Library Kit for Illumina utilizes a proprietary adapter attachment chemistry which minimizes bias and supports inputs as low as 10 pg.
Source: Swift biosciences

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Accel-NGS 2S PCR-Free DNA Library Kit for Illumina

The Accel-NGS 2S PCR-Free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.
Source: Swift biosciences

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ClaSeek Library Preparation Kit, Illumina™ compatible

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input. The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.
Source: Thermo Fisher

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EpiNext™ DNA Library Preparation Kit

The EpiNext™ DNA Library Preparation Kit (Illumina) is a complete set of optimized reagents to carry out a successful DNA library preparation. The kit is suitable for preparing a DNA library for next generation sequencing applications using an Illumina sequencer, which includes genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, bisulfite-seq, and targeted re-sequencing. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) andbarcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.
Source: Epigentek

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EpiNext™ High-Sensitivity DNA Library Preparation Kit

The EpiNext™ High-Sensitivity DNA Library Preparation Kit (Illumina) is a complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications. This kit is suitable for preparing a DNA library using sub-nanogram amounts of DNA input for next generation sequencing applications using an Illumina sequencer. These applications include genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, traditional bisulfite-seq, and targeted re-sequencing. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be constructed quickly with reduced bias.
Source: Epigentek

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Illumina Library Prep Kits

Illumina Library Prep Kits and Barcode Kits for Illumina Multiplexing
Bioo Scientific offers a broad range of optimized Illumina library preparation kits and barcode kits for Illumina multiplexing designed to reduce bias and increase the sensitivity, flexibility and speed of next-generation sequencing. These include kits for construction of DNA-seq, mtDNA-Seq, RNA-seq, methyl-seq, metagenomics and small RNA-seqlibraries. All of the NEXTflex library prep kits have been functionally validated by preparation of a genomic DNA, ChIP DNA, mitochondrial DNA, mRNA or small RNA library, followed by Illumina sequencing.
Source: BiooScientific

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KAPA DNA Library Preparation Kits

KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing.
Source: KAPABIOSYSTEMS

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NEBNext® DNA Library Prep Master Mix Set for Illumina®

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries.
Source: NEB

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NEBNext® Ultra™ DNA Library Prep Kit for Illumina

The NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.
Source: NEB

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NEBNext® mRNA Library Prep Master Mix Set for Illumina®

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries.
Source: NEB

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NEBNext® Small RNA workflow

The novel NEBNext® Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext® Small RNA Library Prep Set for Illumina® (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.
Source: NEB

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NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing.
Source: NEB

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Nextera DNA Library Preparation Kits

Nextera DNA Library Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatible with all Illumina sequencers.
Source: Illumina

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Nextera Mate Pair Sample Preparation Kit

The Nextera Mate Pair Sample Preparation Kit offers the only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation — even in the most complex genomes such as those derived from cancer.
Source: Illumina

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Nextera Rapid Capture Exome and Expanded Exome

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 37 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing. An expanded kit increases coverage to UTRs and miRNA.
Source: Illumina

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Nextera XT DNA Library Preparation Kit

The Nextera XT DNA Library Preparation Kit allows the preparation of sequencing-ready libraries for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.
Source: Illumina

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NEXTflex™ DNA Sequencing Kit

The NEXTflex™ DNA Sequencing Kit is designed to prepare single, paired-end, and multiplexed genomic DNA libraries for sequencing using Illumina® platforms. The enhanced NEXTflex Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. The NEXTflex DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. With these changes to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters, and can expect to see better binding efficiencies.
Source: Bioo Scientific

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NxSeq Long Mate Pair Library Kit

The NxSeq Long Mate Pair Library Kit and Index Kit are compatible with Illumina MiSeq (300, 500, and 600 cycle reagent kits), HiSeq 2500 (250 cycle reagent kit), and NxSeq 500 (300 cycle reagent kit) instruments. The product is not compatible with 50, 75, or 150 cycle reagents kits for any instrument.
Source: Lucigen

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Ovation® Target Capture Module

The Ovation Target Capture Module provides library adaptor-specific blocking reagents to enable target capture workflows with NuGEN library solutions for the Illumina® NGS platforms. Target or sequence capture methods allow researchers to capture genomic regions of interest. The resulting enriched products are utilized for sequencing, resulting in cost reduction, simplified data analysis and increased sample throughput as compared to whole genome sequencing. The Ovation Target Capture Module has been optimized for the SureSelect Target Enrichment System from Agilent Technologies.
Source: Agilent

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QuantSeq-Flex Targeted RNA-Seq Library Prep Kit

The QuantSeq-Flex Kit is a library preparation protocol designed to make Illumina compatible libraries from any RNA sample using custom primers. It is open for development by advanced RNA-Seq users based on their custom needs.
Source: Lexogen

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SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina® sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines key RiboGone and SMART technologies, seamlessly blending efficient rRNA removal and strand-specific library generation in around 5 hours. Because rRNA comprises ~90% of total RNA, effective depletion of these abundant transcripts prior to library generation can lower sequencing costs and improve mapping statistics. The core SMART technology completes a streamlined process that maintains strand information, and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and PCR amplification steps.
Source: Clontech

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TruSeq Custom Amplicon

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. With targeted resequencing, researchers can focus interrogation on key regions of genomic interest. The assay allows researchers to sequence up to 1536 amplicons in a single reaction using a simple workflow. This highly targeted approach offers unparalled efficiency for discovering, validating, and screening genetic variants in areas of research focus.
Source: Illumina

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TruSeq DNA PCR-Free Library Preparation Kits

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes. The kits offer shortened gel-free workflows, the ability to sequence the most challenging regions, and the power to identify the greatest number of variants.
Source: Illumina

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TruSeq Nano DNA Library Preparation Kit

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.
Source: Illumina

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TruSeq Rapid Cluster kits

TruSeq Rapid Cluster kits are the best solution for your cluster generation needs because they enable the unique on-board cluster generation process. On-board cluster generation leverages improved cluster generation chemistry to revolutionize the way you set up your sequencing runs. TruSeq Rapid Cluster kits are designed to be used in the rapid run mode of HiSeq 2500 and 1500 sequencers.
Source: Illumina

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TruSeq RNA Library Preparation Kits

The TruSeq RNA Library Preparation Kits provide a simple, cost-effective solution for generating mRNA-focused libraries from total RNA. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples.
Source: Illumina

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TruSeq Small RNA Sample Preparation Kits

TruSeq Small RNA Sample Preparation Kits provide a simple, cost-effective solution for generating small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing microRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.
Source: Illumina

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TruSeq Stranded mRNA Library Preparation Kit

The TruSeq Stranded mRNA Library Preparation Kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient, and scalable solution for mRNA analysis. Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
Source: Illumina

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TruSeq Stranded Total Library Preparation kit

The TruSeq Stranded Total Library Preparation kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient and scalable solution for total RNA analysis with sequencing.
Source: Illumina

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TruSeq Synthetic Long-Read DNA Library Prep Kit

The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end solution that can be used for genome assembly or genome phasing.
Source: Illumina

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Library Preparation Kits for Ion Torrent

Accel-NGS DNA Library Kit

The Accel-NGS DNA Library Kit for Ion Torrent platforms is the only commercially available kit capable of producing PCR-free libraries with as little as 5 ng of input DNA. PCR-free capability minimizes base composition bias and fidelity issues, while a highly efficient adapter ligation process reduces the input requirements.
Source: Swift biosciences

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GeneRead DNA Library L Core Kit (12)

For 12 reactions: Buffers and reagents for end-repair, ligation and nick-repair, for use with Ion Torrent instruments
Source: Qiagen

Ion Total RNA-Seq Kit for AB Library Builder™ System

The Ion Total RNA-Seq Kit for AB Library Builder™ System combines optimized reagents and protocols for automated preparation of representative cDNA libraries for strand-specific RNA sequencing of all types of RNA species on the Ion Personal Genome Machine (PGM™) and Proton™ sequencers. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing.
Source: Thermo Fisher

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Ion TrueMate™ Plus Library Kit

The Ion TrueMate™ Plus Library Kit works with high-efficiency DNA fragment shotgun sequencing to enable fast, easy, and accurate genome assemblies. This kit comes with a selection of restriction enzymes needed for the generation of mate-pair libraries (2-8 kb inserts) from any genomic DNA. For those customers wishing to select their own restriction enzymes for optimal cleavage of their genome of interest, we offer the Ion TrueMate™ Library Kit, which does not include restriction enzymes.
Source: Thermo Fisher

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Ion Total RNA-Seq Kit v2

The Ion Total RNA-Seq Kit v2 includes the reagents needed to prepare representative cDNA libraries for strand-specific RNA sequencing on the Ion Proton™ or the Ion Personal Genome Machine® (PGM™) systems. The Ion Proton™ System is ideally suited for sequencing the whole transcriptome (ribosomal RNA depleted or polyA), while the Ion PGM™ System is suitable for small RNA, as well as viral and bacterial transcriptomes. Either small RNA (such as microRNA) or whole transcriptome RNA samples can be prepared for next-generation sequencing using the Ion Total RNA-Seq Kit v2.
Source: Thermo Fisher

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Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System

The Ion Xpress™ Plus Fragment Library Kit for AB Library Builder™ System provides a rapid, flexible, and high-throughput solution for library construction upstream of the semiconductor sequencing workflow. This ready-to-use reagent cartridge simplifies next-generation sequencing by enabling automated DNA fragment library preparation for the Personal Genome Machine® and soon the Proton™ sequencing systems.
Source: Thermo Fisher

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Ion PGM and Ion Proton Library Prep and Multiplexing Kits

Ion PGM and Ion Proton Library Prep and Multiplexing Kits
Bioo Scientific offers flexible and robust Ion PGM and Ion Proton library prep and multiplexing kits for Ion Torrent based sequencing platforms. The NEXTflex DNA-Seq library preparation kits offer rapid and efficient Ion Torrent DNA-Seq library prep enabling the construction of DNA libraries from as little as 100 ng of DNA in a streamlined workflow. In addition, the new NEXTflex Cell Free DNA Sequencing Kit for Ion PGM and Ion Proton is optimized to prepare cell free DNA libraries for sequencing using Life Tech’s Ion PGM and Ion Proton sequencing platforms.
Source: BiooScientific

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Ion truemate™ library kit

the Ion truemate™ library kit works with high-efficiency dna fragment shotgun sequencing to enable fast, easy, and accurate genome assemblies. this kit enables the generation of mate-pair libraries (2-8 kb inserts) from any genomic dna, but does not include restriction enzymes. it is intended for those customers who wish to select their own restriction enzymes for optimal cleavage of their genome of interest.
Source: Thermo Fisher

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Thermo Scientific® MuSeek™ Library Preparation Kit

The Thermo Scientific® MuSeek™ Library Preparation Kit provides a fast and simple transposon-based method for preparing high-quality genomic DNA libraries for the Ion PGM™ Sequencer. The kit utilizes MuA transposase enzyme for fragmentation and simultaneous tagging of the target DNA, eliminating the need for separate shearing and adaptor ligation steps. This enables library construction in as little as 80 minutes, resulting in significant savings in time and cost.
Source: Thermo Fisher

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NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™

The NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on the Ion Torrent Sequencer (Life Technologies, Inc.).
Source: NEB

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NEBNext® Fast DNA Library Prep Set for Ion Torrent™

The NEBNext® Fast DNA Library Prep Set for Ion Torrent™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on the Ion Torrent Sequencer (Life Technologies, Inc.).
Source: NEB

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NEXTflex™ DNA Sequencing Kit for Ion PGM™ and Ion Proton™

The NEXTflex™ DNA Sequencing Kit for Ion PGM™ and Ion Proton™ is designed to prepare genomic DNA-Seq or ChIP-Seq NGS libraries for sequencing using Life Technologies’ Ion Torrent PGM and Ion Proton sequencing platforms. The NEXTflex DNA Sequencing Kit for Ion PGM and Ion Proton features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This DNA-seq library prep kit simplifies the Ion Torrent-based library prep workflow by using master mixed reagents and gel-based cleanup. In addition, the option of performing the reactions in a pre-prepared 96-well plate makes this kit more automation friendly than the other available versions.
Source: BiooScientific

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PrepX PGM DNA Library Kit

The PrepX PGM DNA Library Kit provides complete walk-away automation of DNA library prep for Ion Torrent® PGM® sequencers. With 15 to 20 minutes of hands-on time, you can generate 1 to 8 DNA libraries on the Apollo 324 System using our PrepX PGM 200 protocol for 200 bp sequencing or PrepX PGM 100 protocol for 100 bp sequencing. Order the same kit for use with either protocol.
Source: Wafergen

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Library Preparation Kits for Roche 454

GS FLX Titanium Rapid Library Preparation Kit

Kit for processing a DNA sample into a library of single-stranded DNA fragments for subsequent amplification using one of the GS FLX Titanium series emPCR kits, and sequencing on a Genome Sequencer FLX Instrument.
Source: Roche

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NEBNext® DNA Library Prep Master Mix Set for 454™

The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods.
Source: NEB

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NEBNext Quick DNA Library Prep Master Mix Set for 454

The NEBNext Quick DNA Library Prep Master Mix Set for 454 contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries.
Source: NEB

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PrepX™ 454 DNA Library Kit

The PrepX™ 454 DNA Library Kit provides complete walk-away automation of the tedious DNA library prep for Roche 454 GS Junior, FLX, Titanium FLX+ sequencing. With 15 to 20 minutes of hands-on time, the PrepX 454 DNA Library Kit gives you the flexibility to generate 1 to 8 DNA libraries on the Apollo 324™ System using our PrepX 454 FLX protocol for Titanium FLX and GS Junior sequencing, or the PrepX 454 FLX+ protocol for Titanium FLX+ sequencing. Order the same kit for use with either protocol.
Source: Wafergen

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Library Preparation Kits for Solid

NEBNext Small RNA Library Prep Set for SOLiD™

The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible.
Source: NEB

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SOLiD® Total RNA-Seq Kit

The SOLiD® Total RNA-Seq Kit provides protocols and reagents for the construction of strand-specific libraries for small RNAs or the whole transcriptome. Specifically designed for use with the SOLiD® or 5500 Systems, the SOLiD® Total RNA-Seq Kit contains enough reagents to process up to 12 samples.
Source: Thermo Fisher

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Library Preparation Kits for cDNA

cDNA Library Construction Kits

cDNA Library Construction Kits Clontech offers cDNA Library Construction Kits that utilize the SMART cDNA synthesis method to generate full-length cDNA.
Source: Clontech

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Library Preparation Kits for ChIP Seq

NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina®

The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing.
Source: NEB

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PrepX ILM ChiP-Seq Library

PrepX ILM ChiP-Seq Library produces high quality ChIP-Seq libraries in only 70 minutes of run time. It provides complete walk-away automation of DNA library prep for all Illumina sequencers from sub-nanogram ChIP samples. With 15 to 20 minutes of hands-on time, you have the flexibility to generate 1 to 8 DNA libraries on the Apollo 324™ System.
Source: Wafergen

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Library Preparation Kits for FFPE

Ovation® Human FFPE RNA-Seq Multiplex System

The Ovation Human FFPE RNA-Seq Multiplex Systems provide an end-to-end solution for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from human tissues or cell lines. The workflow is equally suitable for good quality RNA from fresh-frozen samples down to 10 ng, or preserved RNA derived from FFPE samples down to 100 ng. Insert Dependent Adaptor Cleavage (InDA-C) technology provides targeted depletion of unwanted high abundance transcripts and results in a significant reduction in sequencing reads derived from human rRNA (12S, 16S, 18S and 28S genes) for more efficient use of sequencing resources.
Source: Nugen

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TransPLEX C-WTA Kit

TransPLEX C-WTA Kit is optimized for the amplification of RNA from difficult samples such as FFPE and serum, providing uniform representation across the entire transcript. The reagent formulations have been designed and optimized to yield a large library from as little as 10 ng of input RNA from fresh tissues and cells or 25 ng from FFPE. The complete TransPLEX C-WTA workflow is performed in a single tube, reducing workflow and minimizing risk of contamination or sample misidentification.
Source: Rubicon Genomics

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Library Preparation Kits for Human Blood

Ovation Human Blood RNA-Seq Library System

The Ovation Human Blood RNA-Seq Library Systems provide an end-to-end solution for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from adult human whole blood. Insert Dependent Adaptor Cleavage (InDA-C) technology provides targeted depletion of unwanted high abundance transcripts and results in a significant reduction in sequencing reads derived from human rRNA (12S, 16S, 18S and 28S genes) and adult human reticulocyte globin (HBA1, HBA2, HBB and HBD genes) for more efficient use of sequencing resources.
Source: Nugen

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Library Preparation Kits for Hydroxymethylation

RRHP™ 5-hmC Library Prep Kit

The RRHP™ 5-hmC Library Prep Kit is the first comprehensive solution for quantitative analysis of genome-wide 5-hmC positions at single base resolution. The Reduced Representation Hydroxymethylcytosine Profiling(RRHP) system is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Shown in the schematic below, fragments lacking glucosylated-5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Positive selection relies on fragments containing 5-hmC being successfully amplified and analyzed.
Source: Zymo Research

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Library Preparation Kits for Mate Pair Cloning

NxSeq® 40 kb Mate Pair Cloning Kit

Each NxSeq® 40 kb Mate Pair Cloning Kit contains: pre-cut, dephosphorylated pNGS FOS vector, CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), DNATerminator End Repair Enzyme Mix and End Repair Buffer, Replicator FOS strain (glycerol stock), 20% Maltose Solution, 1M MgSO4, SM Buffer, Arabinose Induction Solution, Sequencing Primers, and complete protocols.
Source: Lucigen

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Library Preparation Kits for Methyl Sequencing

Accel-NGS Methyl-Seq DNA Library Kit

The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite converted samples and constructs libraries that accurately represent sample base composition.
Source: Swift biosciences

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EpiNext™ Post-Bisulfite DNA Library Preparation Kit

The EpiNext™ Post-Bisulfite DNA Library Preparation Kit is a complete set of optimized reagents to prepare a DNA library -- after successful bisulfite conversion -- for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded(multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.
Source: Epigentek

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EpiNext™ High-Sensitivity Bisulfite-Seq Kit (Illumina)

The EpiNext™ High-Sensitivity Bisulfite-Seq Kit (Illumina) is designed to bisulfite-convert DNA and prepare an Illumina-based library for bisulfite sequencing, all in one kit. Intended applications include whole genome (WGBS), oxidative (oxBS-seq), reduced representation (RRBS), and other bisulfite-next generation sequencing. The optimized protocol and components of the kit allow subnanogram amounts of DNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction in less than 7 hours.
Source: Epigentek

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EpiNext™ RNA Bisulfite-Seq Kit (Illumina)

The EpiNext™ RNA Bisulfite-Seq Kit (Illumina) is a complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded(mutilplexed) library construction using low-nanogram quantities of bisulfite converted RNA.
Source: Epigentek

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Ovation® Ultralow Methyl-Seq Library Systems

Ovation® Ultralow Methyl-Seq Library Systems
The Ovation Ultralow Methyl-Seq Library Systems provide a simple, fast and scalable solution for producing libraries used in conjunction with bisulfite sequencing to analyze DNA methylation and enables methylation studies with a broad range of sample types. The protocol is compatible with whole genome bisulfite sequencing, and can be completed in about nine hours, including bisulfite conversion steps.
Source: Nugen

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Ovation® RRBS Methyl-Seq System

The Ovation RRBS Methyl-Seq System provides a simple, fast and scalable solution for producing reduced representation Methyl-Seq libraries used in conjunction with bisulfite sequencing to analyze DNA methylation. Reduced Representation Bisulfite Sequencing or RRBS is a method that delivers genome-wide quantitative DNA methylation information at single base resolution. Compared to Whole Genome Bisulfite Sequencing at similar depth of coverage, RRBS requires ~50-fold fewer sequencing reads, resulting in a substantial reduction in sequencing costs.
Source: Nugen

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Pico Methyl-Seq™ Library Prep Kit

The Pico Methyl-Seq™ Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as little as 10 pg input DNA (including that derived from FFPE samples), making it ideal for methylation analysis of precious, limited, and target-enriched samples.
Source: Zymo Research

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Library Preparation Kits for mRNA

PrepX RNA-Seq sample and library preparation kits

PrepX RNA-Seq sample and library preparation kits enable the preparation of high-quality directional libraries from 10 ng of total RNA in a single work day. Start with total RNA, and choose between the polyA mRNA isolation and ribo-depletion protocols on the Apollo 324 System. The isolated mRNA is ready for QC or can be directly used for directional library preparation using the PrepX mRNA library preparation protocols. Each run takes 15 to 20 minutes of hands-on time to set up, then press RNA and walk away. The Apollo 324 provides a complete walk-away automation of the tedious library preparation process for RNA-Seq and transcriptome studies.
Source: Wafergen

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QuantSeq 3’ mRNA-Seq Library Prep Kits

The QuantSeq Kit is a library preparation protocol designed to generate Illumina and Ion Torrent compatible libraries of the sequences close to the 3’ end of the polyadenylated RNA.
Source: Lexogen

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SENSE mRNA-Seq Library Prep Kit

The SENSE is a complete strand-specific mRNA-Seq library prep kit for accurate gene expression profiling, transcriptome sequencing, discovery and quantification of antisense transcripts and overlapping genes.
Source: Lexogen

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Library Preparation Kits for Single Cell

Ovation® Single Cell RNA-Seq System

The Ovation Single Cell RNA-Seq System provides an end-to-end solution for strand-specific RNA-Seq library construction using purified total RNA or direct cell lysates. The core technology employed in this product enriches for non-rRNA during cDNA synthesis using proprietary whole transcriptome primers that target non-rRNA sequences in the transcriptome. The workflow can be applied to transcriptomes from a broad range of vertebrate species.
Source: Nugen

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PicoPLEX™ WGA kit

The PicoPLEX™ WGA kit with patented technology* is designed and optimized for amplification of single copy genomic DNA starting with a single cell. The easy-to-use single tube protocol reduces handling errors, dramatically improves time to results and reduces background. PicoPLEX WGA Kit can be also be used with isolated gDNA amounts ranging from less than 6 pg to 50 pg.
Source: Rubicon Genomics

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REPLI-g Single Cell DNA Library Kit

The new REPLI-g Single Cell DNA Library Kit leverages QIAGEN's unique multiple displacement amplification (MDA) technology to overcome these challenges by preparing a sequencing library with high fidelity and minimal bias, while retaining the sample's genomic diversity.
Source: Qiagen

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REPLI-g Single Cell RNA Library Kit

The REPLI-g Single Cell RNA Library Kit leverages QIAGEN's unique MDA technology and GeneRead library construction technology to prepare a sequencing library with high fidelity and minimal bias, while retaining the sample's unique transcriptional profile.
Source: Qiagen

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Strand Specific Library Preparation Kits

Encore® Complete RNA-Seq Library Systems

The Encore® Complete RNA-Seq Library Systems provide an end-to-end solution for strand-specific RNA-Seq library construction using as little as 100 ng of total RNA. The core technology used in this product enriches for non-rRNA in NGS libraries during cDNA synthesis, and can be applied to transcriptomes extracted from a broad range of higher eukaryotes. The first strand cDNA synthesis is carried out using proprietary primers to create double-stranded cDNA which retains RNA strand information.
Source: Nugen

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Ovation® Universal RNA-Seq System

The Ovation Universal RNA-Seq System provides an end-to-end solution for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of tissues or cell lines. The integrated workflow (Figure 1) from input of total RNA to fully constructed library is highly reproducible, and can be completed in approximately 7 hours. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts using a customizable process.
Source: Nugen

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Ovation® Mouse RNA-Seq System

The Ovation RNA-Seq Systems 1-16 for Model Organisms provide an end-to-end workflow for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of model organisms such as mouse, rat, Drosophila and Arabidopsis. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts, and minimizes sequencing reads derived from these transcripts.
Source: Nugen

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Ovation® Rat RNA-Seq System

The Ovation RNA-Seq Systems 1-16 for Model Organisms provide an end-to-end workflow for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of model organisms such as mouse, rat, Drosophila and Arabidopsis. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts, and minimizes sequencing reads derived from these transcripts.
Source: Nugen

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Ovation® Drosophila RNA-Seq System

The Ovation RNA-Seq Systems 1-16 for Model Organisms provide an end-to-end workflow for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of model organisms such as mouse, rat, Drosophila and Arabidopsis. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts, and minimizes sequencing reads derived from these transcripts.
Source: Nugen

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Ovation® Arabidopsis RNA-Seq System

The Ovation RNA-Seq Systems 1-16 for Model Organisms provide an end-to-end workflow for strand-specific RNA-Seq library construction using nanogram amounts of total RNA obtained from a broad range of model organisms such as mouse, rat, Drosophila and Arabidopsis. Insert Dependent Adaptor Cleavage (InDA-C) technology enables targeted depletion of unwanted transcripts, and minimizes sequencing reads derived from these transcripts.
Source: Nugen

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Ovation® Complete Prokaryotic RNA-Seq Library Systems

The Ovation Complete Prokaryotic RNA-Seq Library Systems provide an end-to-end solution for strand-specific RNA-Seq library construction using as little as 100 ng of total RNA obtained from pure cultures of bacteria or mixed populations. The core technology used in this product enriches for non-rRNA in NGS libraries during cDNA synthesis, and can be applied to transcriptomes extracted from a broad range of prokaryotes.
Source: Nugen

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SureSelect Strand Specific RNA Library Preparation Kit

RNA-Seq Agilent's SureSelect Strand Specific RNA Library Preparation Kit is the highest sensitivity, strand-specific method for preparing libraries for mRNA or targeted RNA-seq, enabling a greater understanding of gene regulation.
Source: Agilent

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Solutions for target enrichment can be found under NGS Target Enrichment Panels