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RNA Extraction Kits

This website lists different RNA extraction kits for various sample materials such as blood, cell & tissue, FFPE tissue, microbes, plants, soil stool and virus samples. Some kits are also recommended for DNA and RNA extraction.
Kits are categorized according to the specification indicated by the supplier. Kits categorized as general extraction kits are suitable for extraction of nucleic acids from multiple sample materials.


DNA Extraction Kits   |   Extraction Automatization & Instruments   |   Extraction Supplies


General RNA Extraction Kits ( Multiple Sample Materials)

AccuPrep® DNA/ RNA Extraction Kit

The AccuPrep® DNA/ RNA Extraction Kit is Bioneer’s version of the most popular nucleic acid extraction product type which uses a single spin column with glass micro fiber filters. Bioneer provides various products for high-purity and high-yield Genomic DNA, Plasmid DNA, Viral RNA or fragment DNA. TheAccuPrep® Nano-Plus Plasmid DNA Extraction Kit uses Bioneer’s proprietary cell fragment and protein removal technology (KOR: 10-085043, Nucleic acid separation method and nucleic acid separation mixture using particle material) to reduce plasmid DNA prep time from the usual 30 minutes to a revolutionary 10 minutes. The nano-particles contained within the product form a complex with cellular debris, denatured proteins and chromosomal DNA to form a clear lysate that contains only plasmid DNA within just 1 minute (compared to the traditional 10 minutes), shortening the entire process to a mere 10 minutes.
Source: Bioneer

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ExiPrep™ DNA/ RNA Extraction Kit

The ExiPrep™ DNA/ RNA Extraction Kit can automatically extract high quality nucleic acids from various sample types including blood, animal tissue, cell cultures and plat tissues. This product uses a 'Pre-Loaded Buffer Cartridge System' which provides user convenience and reproducibility by loading all necessary reagents within the kit. The pre-installed protocols are optimized for sample and target nucleic acid type to provide robust results run after run. The silica magnetic particles used in the product have high DNA/RNA binding capacity and were developed using in-house nanoparticle R&D expertise.
Source: Bioneer

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GenEluteTM RNA/ DNA/ Protein purification kit

This kit enables stepwise purification of RNA, DNA and proteins from the same sample from different sample material as cells, tissue, blood, bacteria, fungi or plants. Sigmaaldrich offers different purification solutions for RNA and DNA from different sample material respectively for different nucleic acids like mRNA, microRNA, mammalian total RNA. Additional, they offer different reagents for RNA extraction as TriReagent and RNAzol RT.
Source: Sigmaaldrich

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GET™ Total RNA kit

The GET™ Total RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA Spin Columns. The <60-minute protocol is simple; after homogenization, RNA is bound to the GET™ RNA Spin Columns and washed. The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from the column. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.
Source: Gbiosciences

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PureLink® Viral RNA/DNA Mini Kit

The PureLink® Viral RNA/DNA Mini Kit provides a rapid and efficient method to simultaneously purify viral RNA/DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatants. The PureLink® Viral Mini Kit is specifically designed to isolate high-quality viral nucleic acids from a variety of RNA and DNA viruses within 45 minutes using low elution volumes that allow sensitive downstream analysis. The purified viral RNA/DNA is devoid of proteins and nucleases, and is suitable for use in downstream applications that allow viral detection and genotyping.
Source: Thermo Fisher

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RNAzol®RT

RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. It isolates mRNA and microRNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, the patented reagent allows for the sequential isolation of RNA and DNA.
Source: Gene Copoeia

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RNeasy Plus Kits

RNeasy Plus Kits give you pure total RNA in minutes and eliminate genomic DNA. Optimized protocols for a whole range of samples — even difficult-to-lyse tissues — provide flexibility. Repeatable yields of pure RNA mean certainty for even highly sensitive downstream applications.
Source: Qiagen

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SPLIT RNA Extraction Kit

The SPLIT RNA Extraction Kit enables a fast and highly efficient extraction of RNA that is free of genomic DNA contamination. The RNA can be recovered as total RNA or split into two fractions, large RNA and small RNA, facilitating the analysis of e.g., mRNA and miRNA from the same sample. Thus the RNA obtained is ideal for seamlessly preparing libraries for Next Generation Sequencing of total RNA or its large and small fractions or any other demanding downstream application.
Source: Lexogen

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Tri-Xtract™

Tri-Xtract™ is a convenient, ready-to-use reagent designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridization, in-vitro translation, RNase protection assays and poly (A+) selection. Tri-Xtract™ is a monophasic solution of phenol and guanidine thiocyanate that maintains the integrity of the RNA during cell disruption and homogenization, the addition of chloroform results in the separation of the homogenate into aqueous and organic phases. RNA partitions to the aqueous phase, DNA to the interphase, and proteins to the organic phase. The total RNA is recovered by isopropyl alcohol precipitation. The DNA and proteins can also be recovered by sequential precipitation from the organic phase. Tri-Xtract™ is suitable for the isolation of total RNA, including mRNA, hnRNA, ribosomal RNA and tRNA, from small quantities of tissues (50-100mg) and cells (5 x 106) from human, animal, plant, yeast, bacterial and viral origin. Total RNA is isolated in under an hour and the subsequent isolation of DNA and protein in less than three hours.
Source: Gbiosciences

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TOTALArrest™ RNA kit

The TOTALArrest™ RNA kit isolates total RNA from contaminating DNA, proteins and nucleases using our proprietary binding matrix, pinkRESIN™. The absence of phenol and chloroform extractions make the TOTALArrest™ RNA kit one of the safest methods for isolating and purifying high quality RNA. The 60 minute protocol is simple; after homogenization, RNA is bound to pinkRESIN™ and washed. The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally, RNA is eluted from pinkRESIN™. The eluted RNA is ready for further applications, including Northern blots, dot blots, RNase protective assays or reverse transcription.
Source: Gbiosciences

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Zymo-Spin™ columns

Zymo-Spin™ columns to ensure low-volume elution of ultra-pure RNA that is free of salts and inhibitors. For most sample sources, Zymo Research offers both organic- (TRI Reagent/TRIzol) and non-organic-based methods, allowing the researcher to select the purification method they prefer.
Source: Baseclear

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Blood

NucleoSpin® RNA Blood

Isolation of RNA from fresh or frozen whole blood (human or animal)

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PAXgene Blood miRNA Kit

The PAXgene Blood RNA System consists of a blood collection tube (PAXgene Blood RNA Tube) and a nucleic acid purification kit (PAXgene Blood RNA Kit). It is intended for the collection, transport, and storage of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.
Source: PreAnalytiX

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Cells and Tissue Material

Agencourt RNAdvance Cell v2 kit

RNA Isolation and Purification from Cells The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield. This system reliably delivers high RNA recovery and purity without the need for filtration or centrifugation. The Agencourt RNAdvance Cell v2 chemistry is an automation friendly process for isolating total RNA for utilization in microarray and real time qPCR applications.
Source: Beckmancoulter

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miRNeasy Mini Kit

The miRNeasy Mini Kit enables purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards from all types of animal tissues and cells, including difficult-to-lyse tissues. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required). The miRNeasy Mini Kit is used for low-throughput RNA purification using spin columns.
Source: Qiagen

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NEXTprep™ Small RNA Isolation Kit

The NEXTprep™ Small RNA Isolation Kit is designed for enrichment of small RNA from eukaryotic cells and tissues. The small RNA recovered has been verified as suitable for making small RNA libraries for next-generation sequencing (NGS) using the NEXTflex Small RNA Sequencing Kit. The NEXTprep Small RNA Isolation Kit includes BiooPure™ RNA Extraction Reagent for initial disruption of tissues or cells. BiooPure is a single-phase reagent that contains guanidinium thiocyanate, a powerful chaotropic agent effective for rapidly inactivating nucleases, and phenol, an organic solvent used to denature and separate proteins and DNA from RNA. The kit also includes silica filter spin basket assemblies and tubes for final purification of small RNA by solid phase extraction. The kit allows recovery of small RNAs approximately 100 bases and smaller, with less than ~5% by mass of contamination with larger RNAs.
Source: Bioo Scientific

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NucleoSpin® RNA Plus

New generation for efficient and quick RNA isolation from cells and tissues

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NucleoSpin® miRNA

Parallel isolation of small and large RNA

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NucleoSpin® TriPrep

Parallel isolation of RNA, DNA, and protein from undivided samples

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Agencourt RNAdvance Tissue

The Agencourt RNAdvance Tissue total RNA extraction technology in combination with Beckman Coulter automation offers researchers a true walk away solution for consistent recovery of high quality total RNA in a multi-well format. Utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt RNAdvance Tissue system can extract total RNA from a wide variety of tissues without the hazards and waste removal issues of organic solvents. The Agencourt RNAdvance Tissue system produces high recovery of total RNA from soft, fibrous, and lipid-rich tissues.
Source: Beckmancoulter

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PowerLyzer® UltraClean® Tissue RNA Isolation Kit

The PowerLyzer® UltraClean® Tissue RNA Isolation Kit is designed to provide efficient purification of total RNA from a variety of mammalian tissues or cultured cells.
The BiOstic® Blood Total RNA Isolation Kit provides a way to purify RNA from up to 2 ml of whole blood or 10 million cells using a fast and easy spin column. This method uses a simple hypotonic red blood cell (RBC) lysis to obtain a white blood cell (WBC) pellet for extraction of RNA.
Source: MO BIO

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SV Total RNA Isolation System

The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.
Source: Promega

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ZR-Duet™ DNA/RNA MiniPrep

The ZR-Duet™ DNA/RNA MiniPrep provides a quick method for the isolation of high quality genomic DNA and total RNA from small amounts of cells and tissue. The kit isolates both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol – no extra steps are required! Both DNA and RNA from up to 5x106 cells can be eluted into volumes as little as 25 µl in less than 15 minutes.
Source: Zymo Research

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Circulating RNA

QIAamp Circulating Nucleic Acid Kit

The QIAamp Circulating Nucleic Acid Kit greatly simplifies concentration and purification of free-circulating DNA and RNA from plasma or serum.
Source: Qiagen

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Formalin-fixed, paraffin-embedded (FFPE) tissue samples

Agencourt FormaPure

The Agencourt FormaPure system provides a simple, automation-friendly process for extracting total nucleic acid from Formalin-Fixed, Paraffin-Embedded (FFPE) tissue samples. By utilizing the Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt FormaPure system does not require vacuum filtration, centrifugation, or organic solvents such as phenol or xylene. Researchers can go from paraffin digestion to total nucleic acid extraction in about 4 hours (without setup) for 96 samples in a multi-well format utilizing the Beckman Coulter Biomek NXP or FXP Span-8 workstation.
Source: Beckmancoulter

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Maxwell® 16 LEV RNA FFPE Purification Kit

The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000). The FFPE kit is specifically designed for optimal purification of RNA from 5μm thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximizes RNA yield, eliminating the need to concentrate the extract prior to amplification. Lysates are placed directly into the cartridges, and RNA ready for amplification is obtained in approximately 60 minutes (after proteinase K digest). This kit does not use hazardous xylene, thereby providing a much safer method than many competitive kits. Additionally, quality testing demonstrates virtually no PCR inhibitors in purified samples.
Source: Promega

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NucleoSpin® totalRNA FFPE

Two-in-one kit for the isolation of small (e.g., miRNA) and large RNA from even very limited formalin-fixed, paraffin-embedded samples
Source: Macherey-Nagel

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Prelude FFPE RNA Isolation Module

The Prelude FFPE RNA Isolation Module provides a fast and easy method for isolating total RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The protocol has been optimized to recover total RNA for input into NuGEN's FFPE sample preparation solutions.
Source: Nugen

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truXTRAC™ FFPE DNA and RNA Extraction

truXTRAC™ FFPE DNA and RNA Extraction Covaris truXTRAC is a unique technology that provides the sensitivity and analytical requirement demanded in clinical settings. truXTRAC kits have demonstrated increased yield and provide a robust and reproducible DNA or RNA extraction method for FFPE samples.
Source: Covaris

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Leukocyte

LeukoLOCK™ Total RNA Isolation System

The LeukoLOCK™ Total RNA Isolation System is an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The kit is comprised of a LeukoLOCK™ Fractionation & Stabilization Kit (also available separately; Cat. No. AM1933) and a LeukoLOCK™ Total RNA Isolation Kit.
Source: ThermoFisher

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Microbiome Extraction

PowerMag® Microbiome RNA/DNA Isolation Kit

PowerMag® Microbiome RNA/DNA Isolation Kit Isolate total RNA from stool, gut material, dried feces and contaminated buccal.

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Plant Material

Maxwell® 16 LEV Plant RNA Kit

The Maxwell® 16 LEV Plant RNA Kit is used with the Maxwell® 16 Research Instrument (Cat.# AS2000) to provide an easy method for efficient, automated purification of RNA from plant tissue samples. The Maxwell® 16 Instrument is supplied with preprogrammed purification methods and is designed for use with predispensed reagent cartridges, maximizing simplicity and convenience. The kit provides the reagents required for processing 48 samples and uses prefilled cartridges for purification, maximizing simplicity and convenience. The Maxwell® 16 Instrument can process from 1 to 16 samples in under an hour, and the purified RNA can be used directly in a variety of downstream applications including RT-qPCR, gel electrophoresis, microarrays and Next Gen sequencing.
Source: Promega

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NucleoSpin® RNA Plant

Plant RNA mini spin kit – for all kinds of plant material

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PowerPlant® RNA Isolation Kit

PowerPlant® RNA Isolation Kit For fast and easy isolation of inhibitor-free total RNA

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Soil Samples

PowerSoil® Total RNA Isolation Kit

The RNA PowerSoil® Total RNA Isolation Kit utilizes our novel and proprietary Inhibitor Removal Technology® (IRT) for isolating total RNA from up to 2 grams of soil. RT-PCR and PCR inhibiting substances, including humic substances and the brown color often associated with reverse transcription and PCR inhibition are completely removed. The kit is intended for use with all common soil types, but in particular, with samples that typically contain a high humic substance content, including compost, sediment and manure.
The company offers multiple different extraction kits for different sample material.
Source: MO BIO

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Stool Samples

PowerMicrobiome™ RNA Isolation Kit

PowerMicrobiome™ RNA Isolation Kit Isolate total RNA from stool, gut material, dried feces and contaminated buccal.

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Viral DNA/RNA Extraction

PowerViral® Environmental RNA/DNA Isolation Kit

The PowerViral® Environmental RNA/DNA Isolation Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology® (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool.
Source: MO BIO

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QIAamp Viral RNA Mini Kit

The QIAamp Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids with fast spin-column or vacuum procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube.
Source: Qiagen

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ZR Viral DNA/RNA Kit™

The ZR Viral DNA/RNA Kit™ provide for rapid, single column isolation of high-quality viral nucleic acids from a wide range of biological sources. The kit can be used to successfully isolate viral DNA and RNA from cell-free body fluids as well as cellular suspensions at concentrations ≤ 1 x 105 cells/ml. The procedure employs a single buffer system that facilitates viral particle lysis and allows for the subsequent DNA/RNA binding onto the matrix of the Zymo-Spin™ IIC-XL Column. The nucleic acids are washed then eluted with DNase/RNase-free Water.
Source: Zymo Research

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