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DNA Polymerases/ Kits

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AccuPrime™ DNA polymerases

For accurate PCR performance, AccuPrime™ DNA polymerases set the standard. You’ll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. How does it work? In addition to a powerful DNA polymerase, the AccuPrime™ enzymes include Platinum® anti-DNA polymerase antibodies that provide an automatic hot start, and an accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.
Source: Thermo Fisher

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Accura™ High-Fidelity Polymerase

Accura™ High-Fidelity Polymerase is the perfect solution for your everyday amplification and cloning needs. Amplify your genes for cloning and protein expression applications with confidence while getting the most out of your laboratory budget. The Accura High-Fidelity polymerase is a fusion protein, consisting of a DNA binding domain and a unique, thermostable polymerase derived from a bacteriophage. When used according to recommendations, Accura High-Fidelity polymerase results in robust amplification of normal, high GC% or otherwise difficult templates.
Source: Lucigen

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AmpliTaq Gold® 360

The new AmpliTaq Gold® 360 has the best performance yet and is shipped with a 360 GC enhancer to amplify challenging templates. Use these convenient Hot-Start kits to amplify a full range of even the most challenging targets without compromising sensitivity or specificity.
Source: Thermo Fisher

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EpiQuik Long Taq DNA Polymerase

EpiQuik Long Taq DNA Polymerase is the mixture of two DNA polymerases designed for long PCR. The mixture allows to amplify genomic DNA fragments up to 20 kb and viral DNA fragments up to 40 kb.

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Illustra GenomiPhi V2 DNA Amplification Kit

The illustra GenomiPhi V2 DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 4 to 7 µg DNA can be achieved in less than two hours with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including FTA card lysates, blood and buccal swabs.
GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.
Source: GE Lifescience

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KAPA HiFi DNA Polymerase

KAPA HiFi DNA Polymerase is a novel enzyme engineered specifically for high-fidelity PCR, offering true high fidelity of single-enzyme proofreading polymerases combined with the robustness, sensitivity and ability to amplify long fragments of enzyme blends. KAPA HiFi DNA Polymerase was evolved to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi DNA Polymerase results in significant improvements to yield, sensitivity, target length, and the ability to amplify difficult templates.
Source: KAPA Biosystems

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Lucigen’s PyroPhage 3173 DNA Polymerase

Lucigen’s PyroPhage 3173 DNA Polymerase (patent pending) is the first thermostable enzyme to offer the superior DNA replication performance of a phage replicase. Because all current PCR enzymes are derived from bacterial or archaeal DNA repair polymerases rather than true DNA replicases, they have inherent limitations in DNA replication fidelity, robustness, and processivity. PyroPhage 3173 DNA Polymerase, the first replicase cloned from bacteriophage inhabiting a boiling hot spring, avoids the'se limitations for much more effective DNA amplification.
The thermostability of PyroPhage 3173 DNA Polymerase makes it useful for thermocycling methods, including PCR. The potent 3’-5’ exonuclease (proofreading) activity of the Wild Type enzyme results in extraordinarily high fidelity.
Source: Lucigen

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NGS Library Prep: 2X VeraSeq™ PCR Mix

NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions
Provides efficient, high-fidelity DNA amplification for Next Generation Sequencing library preparation, cloning, and synthetic biology applications.
Source: Enzymatics

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Pfu DNA polymerase

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.
Source: Gbioscience

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phi29 DNA Polymerase

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5' proofreading exonuclease activity.
Source: NEB

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Q5® High-Fidelity DNA Polymerase

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (>100 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Source: NEB

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SequalPrep™ Long PCR Kit

The SequalPrep™ Long PCR Kit with dNTPs provides the highest amplification success rate of any long range PCR kit tested across multiple organisms and platforms for non-optimized amplicons up to 20 kB with little to no optimization. Successful long range PCR with minimal optimization enables efficient PCR enrichment for a variety of applications including medical re-sequencing on next generation sequencing (NGS) platforms.
Source: Thermo Fisher

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Thermo Scientific® Phusion® High-Fidelity DNA Polymerase

Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Reagent selection includes a standalone enzyme, master mix and kit format, as well as a choice of reaction buffers for amplification of difficult templates. Phusion Hot Start Flex DNA Polymerase is available as standalone enzyme or in a master mix format and enables high specificity amplification of a broad range of templates. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. (Phusion® was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.)
Source: NEB

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