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Other Enzymes


Ligase    |    Phosphatase   |    Polymerase    |    Reverse Transcriptase   |  More Enzymes


T4 Polynucleotide Kinase

T4 Polynucleotide Kinase (NEB)

Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
Source: NEB

T4 Polynucleotide Kinase (Promega)

T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
Source: Promega

T4 Polynucleotide Kinase (Sigmaaldrich)

Sigmaaldrich

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T4 Polynucleotide Kinase (ThermoFisher)

Thermo Scientific T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction).
Source: Thermo Fisher Scientific

 

Rapid Amplification of cDNA Ends (RACE)

3' RACE System for Rapid Amplification of cDNA Ends

3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly(A) end (Figure 1). The system is useful for the amplification of rare messages for which little sequence information is available, and for capturing the 3´ end information of mRNA.
Source: Thermo Fisher Scientific

5' RACE System for Rapid Amplification of cDNA Ends

The 5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0, is suitable for rapid amplification of cDNA ends (RACE) between a defined point in the mRNA and the 5´ end. The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA.
Source:Thermo Fisher Scientific

FirstChoice RLM-RACE kit

The FirstChoice® RLM-RACE Kit is designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR. This kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.
Source: Thermo Fisher Scientific

Roche 5′/3′ RACE Kit, 2nd Generation

The 5′/3′ RACE kit (Roche) contains Transcriptor Reverse Transcriptase and recombinant Terminal Transferase. Transcriptor Reverse Transcriptase transcribes full-length cDNA for the highly sensitive and rapid amplification of either 5′ or 3′ cDNA fragments up to 14 kb and, due to its thermostability (up to +65 °C), to work with GC-rich templates with high secondary structure. High sensitivity can be achieved using Transcriptor Reverse Transcriptase, resulting in highly efficient cDNA synthesis and the generation of long RACE products.
Source: Sigmaaldrich

SMARTer RACE 5’/3’ Kit

The SMARTer RACE 5’/3’ Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. The first-strand cDNA generated with this kit can be used directly in 5’- and 3’-RACE PCR reactions.
Source: Clontech

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